Figure 2: Methodology for Reverse Capture Protein Microarray analysis of stage-specific prostate cancer serum samples. (a) serum sample (b) Samples of individual sera from different stages of the disease were diluted as eight serial two-fold dilutions in a 384 well plate (c) samples printed on glass-backed, nitrocellulose (N/C) slides (d) slide image stained for reactivity with monoclonal antibody against PARP and developed by chemiluminescence.