In-house made Reverse Capture protein arrays with serially diluted individual serum samples were exposed to the PARP1 Ab previously used on Clontech arrays, as described in Material & Methods. The PARP1 values were assessed using HARMeans for fluorescence-based signal minus local background intensities. Panel A represents log2-transformed intensities (Y-axis) for each category (NK, SF, AR, and CGI) plotted against sample dilutions (20-40-80- 160, X-axis). Panel B represents serum PARP1 expression values (columns with a trendline, left axis) with corresponding urine Protein / Creatinine Ratio, PCR values (black line, right axis) in the same female patient during ARepisode (fAR 550) and after kidney function stabilization (fSF 677). Panel C represents individual PARP1 HARMean values (bottom) and PCRs (top) with corresponding means in each gender-independent category (NK, SF, AR, CGI). Statistical significance of PCRs and PARP1 was assessed by T-tests (two-tailed distribution and two-sample equal variance) in SF vs. AR or CGI categories.
