Rank Upregulated in Feature Description Score Feature P Fold Change AR Mean AR Std Rest Mean Rest Std 1 Rest P47736 RAP1, GTPase activating protein 1 -3.733 0.010 1.858 0.278 0.068 0.516 0.187 3 AR Q92558 WAS protein family, member 1 3.259 0.012 1.638 0.101 0.021 0.062 0.021 4 Rest P04049 v-raf-1 murine leukemia viral oncogene homolog 1 -3.233 0.022 1.475 0.911 0.208 1.343 0.292 6 Rest P15531 non-metastatic cells 1, protein (NM23A) expressed in -2.981 0.048 1.423 0.613 0.106 0.872 0.239 8 Rest Q99598 translin-associated factor X -2.819 0.026 1.934 0.038 0.018 0.074 0.031 9 Rest O08961 OLF-1/EBF associated zinc finger gene -2.738 0.012 1.335 0.153 0.032 0.205 0.034 10 Rest P51956 NIMA (never in mitosis gene a)-related kinase 3 -2.734 0.028 1.328 0.238 0.049 0.316 0.050 12 Rest P16949 stathmin 1/oncoprotein 18 -2.635 0.026 1.256 0.618 0.050 0.777 0.189 14 Rest P47210 proteasome (prosome, macropain) 26S subunit, ATPase, 5 -2.447 0.032 1.645 0.087 0.021 0.144 0.071 15 Rest P55060 CSE1 chromosome segregation 1-like (yeast) -2.443 0.024 2.104 0.055 0.034 0.116 0.064

Table 2: Candidate gender-independent AR-markers for Acute Rejection in kidney transplant patients. Microarray analysis using protein Ab arrays (Clontech-500) with pooled serum samples from kidney transplantation patients and controls was performed, as described in Material and Methods. Candidate AR-markers were identified in AR vs. Rest comparison (4-classes: NK, SF, AR, and CGI) using Comparative MarkerS election (Gene Pattern). Final selection was made using rank, score, feature P, and fold change.