Rank Upregulated in Feature Description Score Feature P Fold Change CGI Mean CGI Std Rest Mean Rest Std 1 Rest O14920 inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta -3.623 0.008 0.002 0.012 1.276 1.229 0.090 2 Rest Q96NG9 syntaxin binding protein 5 (tomosyn) -3.574 0.006 0.001 0.009 1.267 0.141 0.007 3 Rest Q14186 transcription factor Dp-1 -3.479 0.028 0.017 0.037 1.759 0.265 0.051 4 Rest P28482 mitogen-activated protein kinase 1 -3.382 0.006 0.001 0.009 1.908 0.181 0.071 5 Rest P09874 ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) -3.269 0.050 0.036 0.062 2.036 0.232 0.067 6 Rest Q09028 retinoblastoma binding protein 4 -3.175 0.010 0.004 0.015 1.653 0.101 0.015 8 Rest O95644 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 -2.963 0.010 0.004 0.015 2.105 0.068 0.025 10 Rest Q9NQX3 gephyrin -2.933 0.018 0.009 0.025 1.255 0.965 0.072 12 Rest P42679 megakaryocyte-associated tyrosine kinase -2.746 0.036 0.024 0.046 1.738 0.171 0.046 13 Rest Q07820 myeloid cell leukemia sequence 1 (BCL2-related) -2.513 0.028 0.017 0.037 1.911 0.110 0.038

Table 3: Candidate gender-independent CGI-markers for Chronic Graft Injury in kidney transplant patients. Microarray analysis using protein Ab arrays (Clontech-500) with pooled serum samples from kidney transplantation patients and controls was performed, as described in Material and Methods. Candidate CGI-markers were identified in CGI vs. Rest comparison (4-classes: NK, SF, AR, and CGI) using Comparative Marker Selection (Gene Pattern). Final selection was made using rank, score, feature P, and fold change.