Rank Upreulated in Feature Description Score Feature P FDR (BH) Fold Change AR-CGI Mean AR-CGI Std Rest Mean Rest Std 1 Rest P28482 mitogen-activated protein kinase 1 -3.870 0.004 0.260 1.817 0.216 0.062 0.393 0.113 2 Rest P09874 ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) -3.527 0.004 0.260 2.128 0.263 0.090 0.560 0.220 3 Rest P13624 signal recognition particle 54kDa -3.472 0.008 0.272 1.565 0.099 0.027 0.154 0.036 4 Rest Q14186 transcription factor Dp-1 -3.199 0.004 0.260 1.747 0.302 0.075 0.529 0.185 5 Rest P49918 cyclin-dependent kinase inhibitor 1C (p57, Kip2) -3.107 0.002 0.238 1.842 0.088 0.019 0.162 0.065 6 Rest O14490 dynamin 1-like -3.084 0.002 0.238 1.957 0.050 0.021 0.099 0.039 10 Rest P24864 cyclin E1 -2.879 0.008 0.272 1.729 0.429 0.048 0.741 0.303 11 Rest P42679 megakaryocyte-associated tyrosine kinase -2.868 0.002 0.238 1.832 0.188 0.045 0.343 0.147 14 Rest Q13691 v-ablAbelsonmurineleukemia viral oncogenehomolog 1 -2.678 0.006 0.260 1.649 0.095 0.020 0.156 0.062 15 Rest O95644 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 -2.483 0.006 0.260 1.940 0.084 0.028 0.163 0.086

Table 4: Candidate gender-independent common AR-CGI-markers for Acute Rejection and Chronic Graft Injury in kidney transplant patients. Microarray analysis using protein Ab arrays (Clontech-500) with pooled serum samples from kidney transplantation patients and controls was performed, as described in Material and Methods. Candidate AR-CGI-markers were identified in AR-CGI vs. Rest comparison (3-classes: NK, SF, AR-CGI) using Comparative Marker Selection (Gene Pattern). Final selection was made using rank, score, feature P, and fold change.