Cell Line Rinse Solvent System Ref. GS-CHO, LB01 60% methanol w/ 0.85% (w/v) amm. bicarbonate 100% MeOH [30] Super-CHO, clone C2.8 SPF 0.9% (w/v) NaCl 50% ACN [2] CHO, DG44 line 150 mM NaCl 2:1 (v/v) MeOH:CHCl3 [31] Cord blood lymphocytes None 100% ACN [32] Embryonic kidney, HEK 293 0.9% (w/v) NaCl 50% ACN [33] T lymphoblastic leukemia, Jurkat PBS 80% MeOH [28] Acute myeloid leukemia None 80% MeOH [29]

Table 1: Summary of Metabolite Extraction Systems for Suspension-Cultured Mammalian Cells.