FirstAuthor, Year (PMID) Samples Sample Details Separation MS Machine Model Protein Identification Database
Epiney,2012 [4] (22234358) Secreted Media from primary cultured cytotrophoblasts LC-ESI-MS/MS linear trap quadrupole (LTQ) Orbitrap Velos SwissProt/ trEMBL database
Feng, 2012 [39] (22414876) Tissue 1 x 5g decidual tissue 2DE, MALDI-TOF-MS Voyager-DE STR 4307 SwissProt database via Mascot-Wizard Engine
Gharesi-Fard, 2010 [40] (19954843) Tissue 5 x 500mg samples from each placenta 2DE, MALDI-TOF-TOF N/A NCBI via Mascot Engine
Jin, 2008 [41] (18940332) Tissue 1 x 1cm squared sample from the center of each placenta; LCM LC- 2DE, TOF-TOFMS/ MS Linear trap quadrupole (LTQ) Orbitrap IPI human v3.25 database via Mascot Engine
Kolla, 2012 [42] (22570525) Serum Week 12 blood sample, 9ml iTRAQ labeling, Nano- LC-MALDI-TOF/TOFMS/ MS 4800 Plus MALDITOF/ TOF Analyzer (Applied Biosystems) PANTHER database
Lui, 2011 [43] (21145106) Serum Blood Samples Tris-Glycine SDS-gel, LC-MS/MS Thermo LTQ-Orbitrap mass spectrometer Human IPI database via Mascot engine
Ma, 2013 [44] (24343450) Tissue 1 x 1cm squared sample from the center of maternal face of the placenta; LCM ICAT, 2DE, LC-MS/MS Esquire 3000 ion trap mass spectrometer Geneontology.org
Park, 2011 [45] (21646846) Serum 10ul venous blood before delivery LC-MS/MS LTQ ion-trap mass spectrometer NCBInr database via SEQUEST algorithms
Shi, 2013 [30] (23671712) Tissue 1 x 1cm squared sample from the center of maternal face of the placenta; isolate for mitochondria iTRAQ labeling, nano- LC-MS/MS LTQ-Orbitrap MS Mascot engine
Shin, 2011 [46] (21335933) Tissue 5 x 5 core samples of feto-maternal interface of the placenta 2DE, MALDI-TOF-MS Voyager TM-delayed extraction STR biospectrometry workstation SwissProt or NCBI
Wang, 2013 [28] (24260401) Tissue 1 x 0.5g sample from the maternal face of the placenta, isolate for plasma memb. ICAT, UPLC-MS/MS LTQ-Orbitrap instrument UniProt via Maxquant
Wang, 2013 [29] (24205073) Tissue 1 x 0.5g sample from the maternal face of the placenta ICAT, UPLC-MS/MS LTQ-Orbitrap instrument UniProt via Maxquant
Table 2: Details of samples collected and processing workflow. LCM: Laser Capture Microdissection; LC: Liquid Chromatography; UPLC: Ultra Performance Liquid Chromatography; ESI: Electrospray Ionization; MS: Mass Spectrometry; MALDI: Matrix-Assisted Laser Desorption/Ionization; TOF: Time-of-Flight; iTraq: Isobaric Tag for Relative and Absolute Quantitation