Spota Locus tagb Proteinc MWd pIe Scoref 235 MSC_0027 30S ribosomal protein S6 16094 6.77 109 236 MSC_0027 30S ribosomal protein S6 16094 6.77 107 239 MSC_0027 30S ribosomal protein S6 16094 6.77 66 364 MSC_0027 30S ribosomal protein S6 16094 6.77 124 154 MSC_0051 Hypothetical protein MSC_0051 21553 5.59 47 249 MSC_0053 AhpC/TSA family protein 17397 4.82 46 250 MSC_0053 AhpC/TSA family protein 17397 4.82 84 251 MSC_0053 AhpC/TSA family protein 17397 4.82 114 187 MSC_0079 Prolipoprotein, putative phosphonate ABC transport 50087 9.24 63 189 MSC_0079 Prolipoprotein, putative phosphonate ABC transport 50087 9.24 193 365 MSC_0110 Utp-glucose-1 phosphate uridylyltransferase 32745 5.02 129 196 MSC_0139 Fructose-bisphosphate aldolase class II 32766 6.32 152 262 MSC_0149 Thymidine kinase 23927 5.74 48 360 MSC_0159 Elongation factor G 76137 5.29 227 159 MSC_0160 Translation elongation factor Tu 43277 5.11 190 163 MSC_0160 Translation elongation factor Tu 43277 5.11 138 165 MSC_0160 Translation elongation factor Tu 43277 5.11 209 365 MSC_0160 Translation elongation factor 43277  5.11 92 366 MSC_0160 Translation elongation factor Tu 43277 5.11 72 199 MSC_0218 Guanylate kinase 34253 6.43 143 170 MSC_0263 NADH oxidase 50133 6.12 102 183 MSC_0263 NADH oxidase 50133 6.12 69 184 MSC_0263 NADH oxidase 50133 6.12 150 206 MSC_0264 Lipoate protein ligase 38463 6.17 121 154 MSC_0265 Pyruvate dehydrogenase lipoamide, alfa chain; 41849 5.31 186 155 MSC_0265 Pyruvate dehydrogenase lipoamide alfa chain 41849 5.31 199 205 MSC_0266 Pyruvate dehydrogenase(lipoamide), beta chain 36186 5.93 83 208 MSC_0266 Pyruvate dehydrogenase (lipoamide), beta chain 36186 5.93 117 168 MSC_0267 Dihydrolipoamide S-acetyltransferase 45798 6.22 95 216 MSC_0269 Phosphate acetyltransferase 35673 5.62 69 242 MSC_0274 PTS system ,glucose-specific IIA component 16849 5.35 70 245 MSC_0274 PTS system, glucose-specific IIA component 16849 5.35 84 252 MSC_0274 PTS system,glucose-specific IIA component 16849 5.35 104 238 MSC_0276 Glycerone kinase 22750 6.33 105 237 MSC_0296 Transcription elongation factor 17439 6.77 146 362 MSC_0301 Oxidoreductase 26507 7.74 67 368 MSC_0335 Ribosome-binding factor A 13803 6.62 98 357 MSC_0349 Translation initiation factor IF-2 68760 6.09 106 243 MSC_0353 Hypotetical protein MSC_0353 19505 5.40 87 254 MSC_0451 Elongation factor P 20461 4.96 45 263 MSC_0474 Adenine phosphoribosyltransferase 19281 5.91 126 257 MSC_0493 Copper homeostasis protein 25578 5.44 131 240 MSC_0498 Histidine triad protein 15482 6.06 53 169 MSC_0509 Glyceraldehyde - 3-phosphate dehydrogenase (NADP) 51944 8.35 182 220 MSC_0527 Lipoate-protein ligase A 39777 5.45 122 221 MSC_0527 Lipoate-protein ligase A 39777 5.45 51 207 MSC_0532 L-lactate dehydrogenase 34626 5.86 58 211 MSC_0532 L-lactate dehydrogenase 34626 5.86 119 212 MSC_0532 L-lactate dehydrogenase 34626 5.86 144 241 MSC_0587 Hypothetical protein MSC_0587 16394 5.69 81 367 MSC_0587 Hypothetical protein MSC-0587 16394 5.69 114 143 MSC_0588 Cell division protein FtsZ 41467 4.45 46 363 MSC_0600 Ribosome recycling factor 20799 6.85 80 222 MSC_0607 Elongation factor Ts 32525 5.46 149 185 MSC_0609 Heat shock protein DnaJ(chaperone) 41934 8.40 98 8 MSC_0610 Heat shock protein 70 63915 4.9 268 187 MSC_0678 Phosphoglycerate kinase 44387 6.93 137 191 MSC_0679 Glyceraldehyde 3-phosphate dehydrogenase 36990 7.01 171 192 MSC_0679 Glyceraldehyde-3- phosphate dehydrogenase 36990 7.01 152 200 MSC_0679 Glyceraldehyde-3-phosphate dehydrogenase 36990 7.01 130 217 MSC_0721 DNA-directed RNA polymerase alpha subunit 34989 5.52 229 224 MSC_0747 Glycerone kinase 35842 5.11 61 366 MSC_0747 Glycerone kinase 35842 5.11 48 172 MSC_0761 Aspartyl/glutamyl-tRNA amidotransferase subunit B 56178 6.46 212 230 MSC_0823 Triosephosphate isomerase 27417 6.22 155 232 MSC_0823 Triosephosphate isomerase 27417 6.22 60 262 MSC_0823 Triosephosphate isomerase 27417 6.22 81 28 MSC_0830 Thymidine phosphorylase 48896 5.64 224 144 MSC_0830 Thymidine phosphorylase 48896 5.64 220 148 MSC_0830 Thymidine phosphorylase 48896 5.64 237 150 MSC_0830 Thymidine phosphorylase 48896 5.64 228 226 MSC_0835 Purine nucleoside phophorylase 24496 6.23 103 265 MSC_0835 Purine nucleoside phosphorylase 24496 6.23 120 80 MSC_0893 Uracil phosphoribosyltransferase 25531 8.62 117 225 MSC_0893 Uracil phosphoribosyltransferase 25531 8.62 134 227 MSC_0893 Uracil phosphoribosyltransferase 25531 8.62 119 265 MSC_0893 Uracil phosphoribosyltransferase 25531 8.62 60 190 MSC_0894 Glycine hydroxymethyltransferase 45660 7.17 104 234 MSC_0895 Ribose 5-phosphate isomerase, RpiB 18293 9.20 115 206 MSC_0952 Ribose-phosphate pyrophosphokinase 40104 5.71 81 209 MSC_0952 Ribose-phosphate pyrophosphokinase 40104 5.71 193 213 MSC_0952 Ribose -phosphate pyrophosphokinase 40104 5.71 143 214 MSC_0952 Ribose-phosphate pyrophosphokinase 40104 5.71 186 260 MSC_0962 Transcription antitermination protein nusg 24080 5.22 178 101 MSC_1007 50S ribosomal protein L7/L12 12868 4.95 132 205 MSC_1015 Asparagine synthetase Asn A 38004 6.13 120 355 MSC_1017 Glucose-inhibited division protein A 70697 7.96 196 356 MSC_1017 Glucose-inhibited division protein A 70697 7.96 101 358 MSC_1017 Glucose -inhibited division protein A 70697 7.96 95 Footnotes: aspot number as defined by 2-DE; blocus tag as defined for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2) cprotein annotation as defined for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2) dcalculated molecular mass (Da) based on annotation for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2) ecalculated isoelectric point based on annotation for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2) fMOWSE score obtained through searching MASCOT database for M. mycoides subsp mycoides strain PG1.

Table 1: Proteins from Mycoplasma mycoides subsp. mycoides identified by MALDI-MS/MS following separation by 2-DGE. Proteins are ordered by Locus tab (MSC_nnn) and spot numbers are indicated in the leftmost column. Identified proteins represented by a single spot are indicated in italics; proteins identified through 2-DGE only are indicated in bold italics.