Straina Locus tagb Proteinc N6 MSC_0004 Dimethyladenosine transferase KsgA KH3J MSC_0008 Ribose/Galactose ABC transporter, permease component II N6 MSC_0009 Ribose/Galactose ABC transporter, permease component I N6 MSC_0013 Prolipoprotein N6 MSC_0018 Transcriptional regulator, RpiR family KH3J MSC_0031 Heat shock protein (33 kDa chaperonin) KH3J MSC_0032 Hypothetical protein MSC_0032 KH3J MSC_0034 D-lactate dehydrogenase N6 MSC_0050 Guanosine 5'-monophosphate oxidoreductase N6 MSC_0065 Hypothetical protein MSC_0065 N6 MSC_0066 Seryl-tRNA synthetase KH3J MSC_0085 tRNA modification GTPase N6 MSC_0096 Dephospho-CoA kinase N6 MSC_0105 Endodeoxyribonuclease IV N6 MSC_0133 Hypothetical protein MSC_0133 N6 MSC_0153 Hypothetical protein MSC_0153 N6 MSC_0218 Guanylate kinase KH3J MSC_0219 DNA methylase KH3J MSC_0221 Translation initiation factor IF-3 N6 MSC_0224 Spermidine/putrescine ABC transporter ATP-binding component N6 MSC_0226 Spermidine/putrescine ABC transporter, permease and substrate-binding component N6 MSC_0230 Transposase ISMmy1B KH3J MSC_0235 Leucyl aminopeptidase N6 MSC_0242 Hypothetical protein MSC_0242 N6 MSC_0255 Hypothetical protein MSC_0255 N6 MSC_0256 Hypoxanthine phosphoribosyltransferase KH3J MSC_0257 Glycerol uptake facilitator KH3J MSC_0259 Glycerol-3-phospate oxidase N6 MSC_0272 Pantetheine-phosphate adenylyltransferase N6 MSC_0295 Oxidoreductase N6 MSC_0337 Riboflavin kinase N6 MSC_0347 30S ribosomal protein S15 KH3J MSC_0354 Nitroreductase family protein N6 MSC_0387 Ribosomal large subunit pseudouridine synthase B N6 MSC_0389 Hypothetical protein MSC_0389 KH3J MSC_0405 Hypothetical protein MSC_0405 N6 MSC_0410 DNA-binding protein HU (HB) KH3J MSC_0434 ABC transporter, permease and ATP-binding componen N6 MSC_0448 Hypothetical protein MSC_0448 N6 MSC_0458 ThiJ/PfpI family protein N6 MSC_0461 Hypothetical protein MSC_0461 KH3J MSC_0479 Ribonuclease III KH3J MSC_0500 Hypothetical prolipoprotein N6 MSC_0507 Hypothetical protein MSC_0507 KH3J MSC_0526 NADH dependent flavin oxidoreductase KH3J MSC_0533 N-acetylglucosamine-6-phosphate deacetylase N6 MSC_0544 Dihydrolipoamide dehydrogenase KH3J MSC_0558 Sodium:solute symporter family N6 MSC_0560 50S ribosomal protein L27 N6 MSC_0570 Prolipoprotein N6 MSC_0571 Transposase ISMmy1F KH3J MSC_0577 1-acyl-sn-glycerol-3-phosphate acyltransferase N6 MSC_0581 dCMP deaminase KH3J MSC_0596 Phenylalanine-tRNA ligase alpha subunit KH3J MSC_0598 Hypothetical protein MSC_0598 N6 MSC_0612 Heat-inducible transcription repressor HrcA N6 MSC_0614 Putative hydrolase of the HAD family KH3J MSC_0616 Ham1 family protein N6 MSC_0621 Hypothetical protein MSC_0621 KH3J MSC_0624 Hypothetical protein MSC_0624 N6 MSC_0635 Prolipoprotein N6 MSC_0653 Prolipoprotein N6 MSC_0709 Leucine-tRNA ligase KH3J MSC_0718 ABC transporter, ATP-binding component (vitamin B12) N6 MSC_0723 30S ribosomal protein S13 N6 MSC_0725 Adenylate kinase N6 MSC_0727 50S ribosomal protein L15 N6 MSC_0762 Glutamyl-tRNA amidotransferase subunit A N6 MSC_0764 DNA ligase N6 MSC_0808 Transposase ISMmy1C N6 MSC_0894 Glycine hydroxymethyltransferase N6 MSC_0896 Hypothetical protein MSC_0896 N6 MSC_0916 Hypothetical protein MSC_0916 N6 MSC_0943 Excinuclease ABC subunit A KH3J MSC_0945 Hypothetical protein MSC_0945 N6 MSC_0954 Peptidyl-tRNA hydrolase N6 MSC_0955 50S ribosomal protein L9 N6 MSC_1017 Glucose-inhibited division protein A KH3J MSC_1064 LICA protein Footnotes: astrain in which protein was identified blocus tag as defined for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2) cprotein annotation as defined for M. mycoides subsp. mycoides strain PG1 (accession number NC_005364.2)

Table 3: Proteins detected in only Mycoplasma mycoides subsp. mycoides strain N6 (high pathogenicity) or KH3J (low pathogenicity). Proteins are presented in locus tag (MSC_nnn) order. Proteins detected in 3 of 3 or 2 of 3 biological replicates of strain N6 and in either 1 of 3 (standard text) or 0 of 3 (bold text) biological replicates of strain KH3J are unitalicized. Proteins detected in 3 of 3 or 2 of 3 biological replicates of strain KH3J and in either 1 of 3 (standard text) or 0 of 3 (bold text) biological replicates of strain N6 are presented in italics.