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Figure 1: Extracted Ion Chromatograms of the measured ceramide species by LC/MS. Individual ceramide species were quantified using LC/ESI-MS/MS in the MRM mode on an Agilent 6410 triple quadruple mass spectrometer. Extracted ion chromatograms and m/z transitions for the 12 endogenous ceramide species and the Cer C17:0 internal standard are shown. In the nomenclature (Cer C14:0), the number before the colon refers to length of the carbon chain and the number after the colon to the number of double bonds in the chain. |