Ceramide species Kidney Ceramides Plasma Ceramides Pearson r p value Pearson r p value Cer C14:0 -0.5968 0.0089 0.4498 0.0805 Cer C16:0 -0.5736 0.0128 0.6568 0.0057 Cer C18:0 -0.5964 0.0090 0.5802 0.0185 Cer C20:0 -0.3109 0.2093 0.6876 0.0032 Cer C22:0 -0.4414 0.0667 -0.0714 0.7928 Cer C24:0 -0.5824 0.0112 -0.2878 0.2797 Cer C24:1 -0.6688 0.0024 0.3062 0.2487 Glu-Cer C16:0 -0.9189 <0.0001 0.0826 0.7611 Glu-Cer C18:0 -0.4802 0.0437 0.8837 <0.0001

Table 1: Correlation analysis of ceramide levels and urine ACR. Pearson correlation analysis of 24 h urine albumin:creatinine ratio (ACR) with kidney ceramide levels or plasma ceramide levels from 24 wk old non-diabetic control (db/+) and diabetic (db/db) mice. Significance was defined as p < 0.05.