Gene Symbol Entrez ID Log Fold Change q-value Degs2 70059 -1.28 0 Ugt8a 22239 -1.07 0 Pdgfd 71785 -0.89 0.01 Mvd 192156 -0.60 0.01 Mapk14 26416 -0.42 0.02 Lass5 (Cers5) 71949 -0.38 0.01 Ugcg 22234 -0.36 0.01 Fads3 60527 -0.35 0 Smpd2 20598 -0.35 0.01 Mapk3 26417 0.27 0.02 Sptlc2 20773 0.36 0.04 Lass4 (Cers4) 67260 0.37 0.01 S1pr1 13609 0.43 0.02 Plce1 74055 0.52 0.01 Asah3l (Acer2) 230379 0.62 0 Plch1 269437 1.07 0 Plcl2 224860 1.25 0 Racgap1 26934 1.39 0.02 Smpdl3b 100340 2.83 0.02

Table 3: Significantly altered genes in ceramide metabolism. Gene expression profiling of kidney cortex of 24 wk old db/db diabetic mice and db/+ non-diabetic controls was performed using Affymetrix Mouse Genome 430 2.0 arrays. Genes involved in ceramide synthesis and metabolism were identified and significantly altered genes from kidney cortex of diabetic mice compared to non-diabetic controls are shown. Significance was defined as q < 0.05.