Figure 3: Proteomics NSAF analysis of Mediator core and kinase module subunits identified from HaloTag-Mediator complex isolations and Western blot analysis. A) Biological replicates of pulldowns from Mediator-HaloTag fusions indicated were analysed by LC-MS/MS and shown are averaged NSAF values for all Mediator core and kinase module components from the respective isolations. NSAF values are depicted in three categories; in Dark Blue are values greater than 1 × 10-3, in Blue are values between 1 × 10-3 - 1 × 10-4, and in Light Blue are values less than 1 × 10-4. B) Western blots of HaloTag alone control (HT), HT-MED13 and HT-MED13L complex isolations. HT-MED13 and HT-MED13L samples were diluted 1X, 1:2, 1:4, and 1:8 as indicated. The HT control sample was used without dilution at 1X. Antibodies against MED13, MED13L, MED12, CDK9, CDK19, and MED26 were used to probe HT, HT-MED13, and HT-MED13L samples and results for each are shown in the indicated panels.