Figure 1: 2D-DIGE: Proteins extracted from surgical samples. All protein samples are labeled with different fluorescent dyes. The internal control sample, a mixture of a small portion of all individual samples is labeled by Cy3, and the individual samples are labeled by Cy5. The differently labeled samples are then mixed together. The samples are separated according to both the pH and molecular weight ranges. Gel images are then acquired using laser scanning. Finally, interest protein spots selected using data mining are identified in the intact proteins using a mass spectrometer.