Figure 2: GeLC-MS: Proteins are extracted from cell lines, including normal cell lines and treated cell lines (for example, those treated with siRNA). All protein samples are separated using SDS-PAGE. The gels are sliced into 24 gels in each lane. The cut gels are digested using in-gel trypsinization. The acquired peptides are analyzed using LC-MS to identify proteins. The results obtained from all slices are combined to generate semiquantified data using the Protomap method [35].