Figure 4: Identification of interaction proteins associated with NPM1: Immunoprecipitation (IP) was performed using two Ewing’s cell lines (SKES1 and CHP100) and antibodies (NPM1 and IgG (Santa Cruz, TX)). The IP samples were separated using SDS-PAGE, and the gel images were compared between the NPM1 samples and IgG samples in each cell line. In this study, we identified 20 bands with significantly different densities in the two cell lines. We then identified the proteins included in each band using a mass spectrometer. The identified proteins are listed in Table 1.