Gel band No Cell line name  MW in the gel image(KDa)1) Name Protein Name 2) Molecular Weight 2) Mascot Score 2) 1 SKES1 54 TBA1A_HUMAN Tubulin alpha-1A chain Mass:50956 Score:71 1 SKES1 54 SERA_HUMAN D-3-phosphoglycerate dehydrogenase Mass:57538 Score:45 2 SKES1 52 VIME_HUMAN Vimentin  Mass:53690 Score:206 2 SKES1 52 TBB2C_HUMAN Tubulin beta-2C chain Mass:50367 Score:128 2 SKES1 52 TBA1A_HUMAN Tubulin alpha-1A chain Mass:50956 Score:117 2 SKES1 52 GFAP_HUMAN Glial fibrillary acidic protein Mass:49921 Score:95 2 SKES1 52 ATPA_HUMAN ATP synthase subunit alpha, mitochondrial Mass:59856 Score:63 3 SKES1 49 TBB5_HUMAN Tubulin beta chain  Mass:50207 Score:372 3 SKES1 49 ATPA_HUMAN ATP synthase subunit alpha, mitochondrial Mass:59856 Score:164 3 SKES1 49 ATPB_HUMAN ATP synthase subunit beta, mitochondrial  Mass:56525 Score:143 4 SKES1 36 NPM_HUMAN Nucleophosmin Mass:32768 Score:134 4 SKES1 36 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1 Mass:37478 Score:99 4 SKES1 36 PCBP2_HUMAN Poly(rC)-binding protein 2 Mass:39053 Score:73 4 SKES1 36 RA1L3_HUMAN Putative heterogeneous nuclear ribonucleoprotein A1-like protein 3 Mass:34415 Score:48 4 SKES1 36 ROA3_HUMAN Heterogeneous nuclear ribonucleoprotein A3  Mass:39855 Score:44 5 SKES1 35 G3P_HUMAN Glyceraldehyde-3-phosphate dehydrogenase Mass:36244 Score:190 5 SKES1 35 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1  Mass:37478 Score:184 5 SKES1 35 CAZA1_HUMAN F-actin-capping protein subunit alpha-1  Mass:33115 Score:135 5 SKES1 35 HNRH3_HUMAN Heterogeneous nuclear ribonucleoprotein H3 Mass:36974 Score:75 5 SKES1 35 PCBP2_HUMAN Poly(rC)-binding protein 2 Mass:39053 Score:57 6 SKES1 33 ROA1_HUMAN Heterogeneous nuclear ribonucleoprotein A1  Mass:38964 Score:547 6 SKES1 33 PHB2_HUMAN Prohibitin-2  Mass:33276 Score:279 6 SKES1 33 LDHA_HUMAN L-lactate dehydrogenase A chain  Mass:37021 Score:93 6 SKES1 33 ROA0_HUMAN Heterogeneous nuclear ribonucleoprotein A0  Mass:31035 Score:80 6 SKES1 33 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1 Mass:37478 Score:77 6 SKES1 33 VDAC2_HUMAN Voltage-dependent anion-selective channel protein 2  Mass:32186 Score:71 7 SKES1 30 EFHD2_HUMAN EF-hand domain-containing protein D2 Mass:26823 Score:116 7 SKES1 30 RS3_HUMAN 40S ribosomal protein S3  Mass:26885 Score:105 7 SKES1 30 RL8_HUMAN 60S ribosomal protein L8  Mass:28291 Score:66 7 SKES1 30 CAPZB_HUMAN F-actin-capping protein subunit beta Mass:31686 Score:50 7 SKES1 30 SFR2B_HUMAN Splicing factor, arginine/serine-rich 2B  Mass:32410 Score:46 7 SKES1 30 RS2_HUMAN 40S ribosomal protein S2 Mass:31660 Score:42 7 SKES1 30 RFA2_HUMAN Replication protein A 32 kDa subunit  Mass:29371 Score:39 8 SKES1 26 TPIS_HUMAN Triosephosphate isomerase Mass:27008 Score:74 8 SKES1 26 BAP31_HUMAN B-cell receptor-associated protein 31 Mass:28045 Score:72 8 SKES1 26 RAB21_HUMAN Ras-related protein Rab-21 Mass:24830 Score:62 8 SKES1 26 RALA_HUMAN Ras-related protein Ral-A  Mass:23765 Score:57 8 SKES1 26 SNP23_HUMAN Synaptosomal-associated protein 23  Mass:23766 Score:38 8 SKES1 26 RL19_HUMAN 60S ribosomal protein L19 Mass:23593 Score:35 9 SKES1 16 GAPR1_HUMAN Golgi-associated plant pathogenesis-related protein 1 Mass:17350 Score:178 9 SKES1 16 H4_HUMAN Histone H4  Mass:11360 Score:176 9 SKES1 16 H2B1C_HUMAN Histone H2B type 1-C/E/F/G/I  Mass:13811 Score:174 9 SKES1 16 H2B1B_HUMAN Histone H2B type 1-B  Mass:13942 Score:160 9 SKES1 16 PPIA_HUMAN Peptidyl-prolyl cis-trans isomerase A Mass:18285 Score:107 9 SKES1 16 H31T_HUMAN Histone H3.1t Mass:15641 Score:84 9 SKES1 16 DCD_HUMAN Dermcidin  Mass:11419 Score:80 9 SKES1 16 RL31_HUMAN 60S ribosomal protein L31  Mass:14454 Score:63 9 SKES1 16 RLA2_HUMAN 60S acidic ribosomal protein P2  Mass:11658 Score:53 9 SKES1 16 H2A1A_HUMAN Histone H2A type 1-A  Mass:14225 Score:53 9 SKES1 16 MYL6_HUMAN Myosin light polypeptide 6  Mass:17132 Score:47 9 SKES1 16 RL35_HUMAN 60S ribosomal protein L35  Mass:14543 Score:42 10 CHP100 68 PLAK_HUMAN Junction plakoglobin Mass:82572 Score:419 10 CHP100 68 KPRP_HUMAN Keratinocyte proline-rich protein  Mass:67929 Score:47 11 CHP100 54 TBA1A_HUMAN Tubulin alpha-1A chain  Mass:50956 Score:120 11 CHP100 54 HNRPK_HUMAN Heterogeneous nuclear ribonucleoprotein K Mass:51300 Score:87 11 CHP100 54 SPB12_HUMAN Serpin B12 Mass:46744 Score:60 12 CHP100 53 VIME_HUMAN Vimentin  Mass:53690 Score:264 12 CHP100 53 TBA1A_HUMAN Tubulin alpha-1A chain  Mass:50956 Score:248 12 CHP100 53 TBB5_HUMAN Tubulin beta chain  Mass:50207 Score:63 12 CHP100 53 RBBP4_HUMAN Histone-binding protein RBBP4 Mass:47981 Score:58 12 CHP100 53 ATPA_HUMAN ATP synthase subunit alpha, mitochondrial Mass:59856 Score:54 12 CHP100 53 GFAP_HUMAN Glial fibrillary acidic protein  Mass:49921 Score:38 13 CHP100 49 ATPB_HUMAN ATP synthase subunit beta, mitochondrial Mass:56525 Score:364 13 CHP100 49 ATPA_HUMAN ATP synthase subunit alpha, mitochondrial  Mass:59856 Score:196 13 CHP100 49 HNRH1_HUMAN Heterogeneous nuclear ribonucleoprotein H  Mass:49554 Score:95 14 CHP100 37 NPM_HUMAN Nucleophosmin Mass:32768 Score:106 14 CHP100 37 PCBP2_HUMAN Poly(rC)-binding protein 2 Mass:39053 Score:46 14 CHP100 37 ARGI1_HUMAN Arginase-1 Mass:34926 Score:37 15 CHP100 36 NPM_HUMAN Nucleophosmin  Mass:32768 Score:68 15 CHP100 36 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1  Mass:37478 Score:54 15 CHP100 36 ROA3_HUMAN Heterogeneous nuclear ribonucleoprotein A3  Mass:39855 Score:40 16 CHP100 33 ROA1_HUMAN Heterogeneous nuclear ribonucleoprotein A1  Mass:38964 Score:268 16 CHP100 33 PHB2_HUMAN Prohibitin-2 Mass:33276 Score:227 16 CHP100 33 ROA0_HUMAN Heterogeneous nuclear ribonucleoprotein A0  Mass:31035 Score:112 16 CHP100 33 LDHA_HUMAN L-lactate dehydrogenase A chain Mass:37021 Score:67 16 CHP100 33 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1  Mass:37478 Score:42 17 CHP100 30 TPM3_HUMAN Tropomyosin alpha-3 chain  Mass:32870 Score:115 17 CHP100 30 VDAC1_HUMAN Voltage-dependent anion-selective channel protein 1  Mass:30896 Score:38 17 CHP100 30 VDAC3_HUMAN Voltage-dependent anion-selective channel protein 3 Mass:31066 Score:38 17 CHP100 30 MTCH2_HUMAN Mitochondrial carrier homolog 2  Mass:34090 Score:36 18 CHP100 29 RS3_HUMAN 40S ribosomal protein S3  Mass:26885 Score:92 18 CHP100 29 EFHD2_HUMAN EF-hand domain-containing protein D2  Mass:26823 Score:71 18 CHP100 29 CAPZB_HUMAN F-actin-capping protein subunit beta Mass:31686 Score:57 18 CHP100 29 RFA2_HUMAN Replication protein A 32 kDa subunit  Mass:29371 Score:39 19 CHP100 27 PHB_HUMAN Prohibitin Mass:29857 Score:173 19 CHP100 27 ADT3_HUMAN ADP/ATP translocase 3  Mass:33129 Score:164 19 CHP100 27 ADT2_HUMAN ADP/ATP translocase 2 Mass:33158 Score:140 19 CHP100 27 RL7_HUMAN 60S ribosomal protein L7  Mass:29278 Score:60 19 CHP100 27 1433B_HUMAN 14-3-3 protein beta/alpha  Mass:28207 Score:37 19 CHP100 27 1433S_HUMAN 14-3-3 protein sigma Mass:27899 Score:37 20 CHP100 26 CHCH3_HUMAN Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 Mass:26491 Score:101 20 CHP100 26 SNP23_HUMAN Synaptosomal-associated protein 23  Mass:23766 Score:71 20 CHP100 26 TPIS_HUMAN Triosephosphate isomerase  Mass:27008 Score:52 20 CHP100 26 RL19_HUMAN 60S ribosomal protein L19  Mass:23593 Score:43 20 CHP100 26 RALA_HUMAN Ras-related protein Ral-A  Mass:23765 Score:41 20 CHP100 26 BAP31_HUMAN B-cell receptor-associated protein 31 Mass:28045 Score:39 1) MW: Molecular Weight 2) Mascot score for the identified proteins based on the peptide ions score (p< 0.05) (http://www.matrixscience.com)

Table 1: Protein list of interaction proteins associated with NPM1.