| Approach |
Advantages |
Drawbacks |
| AQUA platform |
-High sensititvity (fluorescence-based method) |
-Quality of antibodies |
| |
-Indicates the subcellular location of the protein |
-Cost |
| |
-Accurate quantitative scoring |
|
| Immunohistochemistry |
-Low cost |
-Quality and cross-reactivity of antibodies |
| |
-In situ analysis of specific proteins |
-No reliable quantitative scores |
| MALDI Imaging |
-In situ imaging |
-Technically painstaking |
| |
-Allows predicting the protein subcellular location |
-High cost |
| Protein microarrays |
-In situ protein screening on a large scale |
-Quality of antibodies |
| Tissue Microarrays |
-Screening of a wide of samples |
-Poor pathological evaluation |
| |
|
-Difficult assembly of individual arrays |
| 2D-Electrophoresis |
-Detection of several proteins in a single run |
-Restricted capacity of proten separation |
| |
-Allows identifying protein post-translational modifications |
|
| Mass spectrometry |
- Identification and quantitation of proteins |
-Technical complexity |
| |
-Sensitivity and specificity |
-Cost is not very acessible |
| |
-High throughput screening of samples |
|
| ELISA |
-Analysis of large sample datasets |
-Limit of detection |
| |
-Low-cost technology |
|
| Western Blotting |
- Easy implementation |
-Not always reprodutible |
