| Proteomic approach |
Technical Considerations |
| SERological proteomic analysis (SERPA) |
Allows identifying specific autoantibodies produced in response to tumor antigens |
| Proteomic Phosphoprofiling |
Employs the immunoaffinity precipitation strategy in association with mass spectrometry stable isotope labeling by amino acids in cell culture (SILAC) |
| OxiCAT Thiol-mapping proteome |
Identifies the redox-sensitive cysteine residues of proteins by using a combined mass spectrometry global approach with isotope-coded affinity tag (ICAT) technology in association with the thiol-trapping technique, after the modification of the accessible cysteine residues by the thiol-trapping reagent iodoacetamide |
| Activity-based profiling (ABPP) platforms |
ABPP are specific probes that link covalently to a given protein (enzyme) by electrophilic and photoreactive groups and are analyzed in association with gel-based and LC-MS methods |
| |
Useful to metadegradomics studies. |
| TMA-AQUA platform |
This tissue microarray analysis allows the in situ large-scale identification of specific targets by fluorescent immunohistochemistry. |
| Imaging mass-spectrometry (IMS) |
Enables analyzing intact tissue samples without sample destruction. IMS allow visualizing the spatial location of proteins in a given sample based on the molecular mass of the molecule. This strategy includes a range of technologies, such as the secondary ion mass spectrometry (SIMS), MALDI imaging and desorption electrospray ionization (DESI). |
| Aptamer-based strategy |
Aptamers selectively bind specific proteins with high affinity, presented as complex high-performance proteomic arrays |
