Figure 4: (A) a 90 minutes HPLC TIC. (B) At elution time point ~20 minutes, a MS spectrum was recorded. One of the peaks (circled) with m/z of 574.438, double charged (insert), was selected for fragmentation and produced a MS/MS spectrum (C). Analysis of the fragment b and y ions led to identification of the peptide LVGGDNLCSGR, which is part of human CD5 antigen-like protein.