Figure 1: Overview of the experimental workflow. Pooled CSF was divided into equal aliquots. Each aliquot was subjected to pre-fractionation devices as follows: Seppro® IgY14, HLB Oasis®, PHM-L LIPOSORB™ or was not subjected to pre-fractionation: unfractionated CSF. Pre-fractionations were performed in triplicate and LC-MS/MS analytical step in duplicate.