Figure 2: A: The total number of LC-MS/MS protein identifications (IDs) obtained with Proteome Discoverer TM (Thermo Scientific) after each prefractionation step (Seppro® IgY14, HLB Oasis®, PHM-L LIPOSORB™) was compared to that of unfractionated CSF. We observed a significant difference for OASIS® HLB and Liposorb™ with 28% more and 48% fewer proteins identified respectively. Statistical significance was tested using unpaired Student’s T-test; statistically highly significant value was set at P<0.001. B: The total number of LC-MS/MS PSM (Peptide Spectral Match) obtained for each pre-fractionation (Seppro® IgY14, HLB Oasis®, PHM-L LIPOSORB™) compared to unfractionated CSF. Both OASIS® HLB and Liposorb™ resulted in significantly higher values. Statistical significance was tested using an unpaired Student’s t-test; statistically highly significant value was set at P<0.001.