| # |
Protein |
Calculated
monoisotopic mass [M+H]+ |
Amino acid number
(N-/ C-terminus) |
Ions
score |
Swiss-prot
accession
number |
Peptide sequence |
| 1 |
Alpha-1-antichymotrypsin |
4352.34 |
387-423 |
102 |
P01011 |
VETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA |
| 2 |
Alpha-1-antitrypsin |
2390.26 |
398-418 |
107 |
P01009 |
MIEQNTKSPLFMGKVVNPTQK |
| 3 |
Alpha-2-HS-glycoprotein |
2739.53 |
341-367 |
85 |
P02765 |
TVVQPSVGAAAGPVVPPCPGRIRHFKV |
| 4 |
Alpha-2-HS-glycoprotein |
2858.42 |
341-367 |
77 |
P02765 |
TVVQPSVGAAAGPVVPPCPGRIRHFKV (S-cysteinylation at Cys358) |
| 5 |
Coagulation factor XIII subunit A |
3949.98 |
2-38 |
58 |
P00488 |
SETSRTAFGGRRAVPPNNSNAAEDDLPTVELQGVVPR(N-acetylation at Ser2) |
| 6 |
Fibrinogen alpha chain |
1465.66 |
21-35 |
109 |
P02671 |
DSGEGDFLAEGGGVR |
| 7 |
Fibrinogen alpha chain |
1616.66 |
20-35 |
110 |
P02671 |
ADSGEGDFLAEGGGVR (phosphorylation at Ser22) |
| 8 |
Fibrinogen alpha chain |
2768.23 |
576-600 |
90 |
P02671 |
SSSYSKQFTSSTSYNRGDSTFESKS |
| 9 |
Fibrinogen alpha chain |
5078.35 |
528-574 |
22 |
P02671 |
TFPGFFSPMLGEFVSETESRGSESGI- FTNTKESSSHHPGIAEFPSRG
(oxidation at Met536) |
| 10 |
Platelet factor 4 |
7759.18*1 |
32-101 |
- |
P02776 |
EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLI-ATLKNGRKICLDLQAPLYKKIIKKLLES*2 |
| 11 |
Prothrombin |
4089.02 |
328-363 |
169 |
P00734 |
TFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGR |
| 12 |
Vasodilator-stimulated phosphoprotein |
3622.78 |
128-160 |
61 |
P50552 |
SVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAG |
| 13 |
Vinculin |
4038.05 |
859-895 |
35 |
P18206 |
APPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVIN |
| 14 |
Vinculin |
4152.99 |
871-906 |
34 |
P18206 |
PPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLH
(oxidation at Met899 and Met900) |
| *1 Detected as a doubly charged ion on BLOTCHIP-MS peptide profiling. |
| *2 Although only partial MS/MS spectra was obtained, mass of the whole chain was identical to the calculated value. |
Characteristics of the 14 identified peptides are shown. MALDI-TOF/TOF and LC-MS/MS peptide sequencing analyses were performed using partially purified serum
peptides after reverse-phase chromatography. Some of the identified peptides were modified (see details in manuscript).