| # |
Protein |
Calculated
monoisotopic mass [M+H]+ |
AUC |
Sensitivity
(%) |
Specificity
(%) |
Cutoff |
Fold change
(CRC/Control) |
ClinProTools
p-value*1 |
| 1 |
Alpha-1-antichymotrypsin |
4352.34 |
0.690 |
60 |
71 |
10,723 |
0.67 |
< 0.000001 |
| 2 |
Alpha-1-antitrypsin |
2390.26 |
0.685 |
74 |
56 |
4,532 |
0.83 |
0.000030 |
| 3 |
Alpha-2-HS-glycoprotein |
2739.53 |
0.742 |
43 |
94 |
54,162 |
0.75 |
< 0.000001 |
| 4 |
Alpha-2-HS-glycoprotein |
2858.42 |
0.754 |
75 |
70 |
58,384 |
0.69 |
< 0.000001 |
| 5 |
Coagulation factor XIII subunit A |
3949.98 |
0.803 |
76 |
73 |
66,666 |
0.62 |
< 0.000001 |
| 6 |
Fibrinogen alpha chain |
1465.66 |
0.583 |
35 |
83 |
182,168 |
1.08 |
0.000517 |
| 7 |
Fibrinogen alpha chain |
1616.66 |
0.571 |
51 |
67 |
52,321 |
1.22 |
0.006910 |
| 8 |
Fibrinogen alpha chain |
2768.23 |
0.589 |
57 |
62 |
28,522 |
0.81 |
0.004350 |
| 9 |
Fibrinogen alpha chain |
5078.35 |
0.647 |
71 |
54 |
20,407 |
0.84 |
0.000004 |
| 10 |
Platelet factor 4 |
7759.18 |
0.567 |
81 |
37 |
24,139 |
0.97 |
0.005640 |
| 11 |
Prothrombin |
4089.02 |
0.549 |
43 |
75 |
7,796 |
0.98 |
0.012600 |
| 12 |
Vasodilator-stimulated phosphoprotein |
3622.78 |
0.712 |
56 |
81 |
3,460 |
1.68 |
< 0.000001 |
| 13 |
Vinculin |
4038.05 |
0.662 |
50 |
78 |
2,112 |
2.97 |
0.024400 |
| 14 |
Vinculin |
4152.99 |
0.636 |
67 |
57 |
9,657 |
1.80 |
0.000001 |
| *1 Wilcoxon rank-sum test. p-value< 0.05 was considered statistically significant. |
|
|
|
BLOTCHIP®-MS analysis of subject serum detected 159 statistically significant peaks, which were differentially expressed in CRC patients and controls. Shapes of all
the peaks obtained by the statistical analyses were examined by manual inspection to obtain 31 peaks that were sharp and strong. From these, 14 peptides were finally
selected as biomarker candidate peptides for CRC diagnosis. Fold changes indicate the ratio of median values (CRC patients/controls). P-values of the Wilcoxon rank-sum
tests were calculated using ClinProTools 2.2. |
