Figure 2: Immuno-blot analysis of lipid rafts isolated from MEC1 cells. (A) Sucrose gradient centrifugation for isolation of LR. The tube was fractionated from top to bottom, an arrow indicates the LR band. (B) Immuno-blot analysis using antibodies against the lipid raft markers Lyn, caveolin-1 and ganglioside M1 (GM1), the plasma membrane marker, transferrin receptor (CD71) and the cytoplasmic marker, actin. The numbers indicate fractions collected. Fractions 1-5 showed the highest levels of lipid raft markers with low levels of markers for the plasma membrane and cytoplasm.