Distance between head and tail  Radius   Distance between head and tail Radius   Distance between head and tail Radius Distance between head and tail of linkers VH1 53.6011 34.7554 VL1 46.7338 30.0575 VHL1 45.6794 216.5253 10.1888 VH2 56.2322 38.7563 VL2 43.6335 27.6651 VHL2 45.6783 216.5592 14.0992 VH3 55.6829 39.5128 VL3 43.6429 27.6653 VHL3 45.6783 216.8067 11.7021 VH4 50.1406 37.4041 VL4 43.6302 27.6654 VHL4 45.6783 215.9425 27.8454 VH5 45.9529 28.5646 VL5 43.6084 27.6612 VHL5 45.6782 216.3861 14.2882 VH6 56.1668 37.7859 VL6 43.6321 27.6654 VHL6 45.6777 217.4843 12.2691 VH7 48.304 31.8512 VL7 43.6263 27.6624 VHL7 45.6782 217.0619 12.7475 VH8 45.406 30.8712 VL8 43.6034 27.6596 VHL8 45.6803 216.3476 26.4597 VH 39.9954 41.8673 VL 43.9975 29.1371

Table 2: Distance between head and tail and radius of VH and VL before and after adding Linkers.