Figure 1: An overview of general methods used for protein isolation and identification in seminal plasma and spermatozoa. Abbreviations: 2D-DIGE: 2 Dimensional-Differential In gel electrophoresis; 2DGE: 2 Dimensional-gel electrophoresis; LC-MS/MS: Liquid chromatographytandem mass spectrometry; MALDI-TOF: Matrix Assisted Laser Desorption Ionization-Time of Flight MASCOT: A tandem mass spectrometry data analysis program from Matrix Science, which is used for protein identification; OS: Oxidative stress; ROS: Reactive oxygen species; SEQUEST: A tandem mass spectrometry data analysis program used for protein identification; TAC: Total antioxidant capacity.