All active compounds interact in the same general orientation in the nucleotide binding site. The residues involved in significant interactions with inhibitors are shown (grey sticks) with polar interactions indicated as yellow dashed lines. The schematic 2D representations shown in the right column were created by LigPlot+ v1.4.2 [13] with hydrophobic interactions shown as red semi-circles with lines radiating out in the direction of the interaction. (a) Amiloride (magenta carbons); (b) DMA (orange carbons); (c) EIPA (cyan carbons); (d) HMA (green carbons); (e) Benzamil (yellow carbons); (f) DCB (peach carbons); and (g) HMB (purple carbons).
