SPECIES GENOME SIZE CRISPR POSITION DIRECT REPEATS DR LENGTH NO OF SPACERS CRISPR LENGTH % IN GENOME START END Methanosarcina barkeri str. Fusaro 4837408 1967688 1967935 GTCAAGCCTTTTTTGAAAAGGGTTG 25 3 247 0.01 3090113 3090480 CTACCACCAGAAATTGGGAAACT 23 5 367 0.01 Methanosarcina acetivorans str. C2A 5751492 1779366 1779592 ATAATATTGCAAATTCAAACAGG 23 3 226 0.00 Methanosaeta thermophila PT 1879471   677280   677982 GTCGAAGAGCGAGTTCCAGGAAAACAAGGATTGAAAC 37 9 702 0.04 Methanosaeta harundinacea 6Ac 2559043 1830362 1833302 CTATCCATGGCTGAAAAGTCGTGGCCCCATTGAAAC 36 39 2940 0.11 Methanoregula boonei 6A8 2542943 408294 408879 GACATCATCATCATGCAGTCGCACATGGACTTCATCATGG 40 6 585 0.02 Methanosphaerula palustris E1-9c 2922917 1642478 1642689 CGGTTCATCCCCACGCTTGTGGGGAACTC 29 3 211 0.01 1642843 1649035 101 6192 0.21 Methanoplanus petrolearius DSM 11571 2843290 1554943 1555291 TATCATCCGCGTTCACCAACTGTTCGGC 28 4 348 0.01 Methanoculleus marisnigri JR1 2478101 1554414 1554604 TCCGGGGTTTCCCGGGGCGTCTCCTC 26 3 190 0.01 Methanococcus aeolicus Nankai-3 1569500 86886 87164 ATAACCATAAATGGAAATGCAGGA 24 4 278 0.02 Methanobrevibacter ruminantium M1 2937203 592392 592735 TGTTGTTTGTGAATGTGTTGTTTATTATCTCTCC 34 4 343 0.01 592857 593121 3 264 0.01 Methanobacterium sp. SWAN-1 2546541 785808 786234 AATTCAAATAACAACACAATATCCGGAAACA 31 6 426 0.01 Caldivirga maquilingensis IC-167 2077567 232508 232870 CTTTCTAATCCCTTTTGGGATTTTC 25 5 362 0.02 1583088 1583382 25 294 0.01 288199 288427 AACTTTCTAATCCCTTTTGGGATTTTC 27 3 228 0.01 1258730 1259356 GAAAATCCCAAAAGGGATTAGAAAG 25 9 626 0.03 1588509 1589276 11 767 0.04 1611612 1612046 6 434 0.02 1700150 1700375 CTTTCTAATCCCTCTTGGGATTTTCT 26 3 225 0.01 Desulfurococcus fermentans DSM 16532 1384116 973322 974943 CTTTCAATTCTTTCTATTGTATTC 24 24 1621 0.12 Pyrobaculum aerophilum str. IM2 2222430 268866 269081 GACGAAACAAATCAAAGAATTGAC 24 3 215 0.01 Sulfolobus solfataricus 98/2 2668974 2054517 2061910 GATTAATCCCAAAAGGAATTGAAAG 25 116 7393 0.28 2076433 2080895 CTTTCAATTCCTTTTGGGATTAATC 25 70 4462 0.17 2092271 2100245 GATAATCTCTTATAGAATTGAAAG 24 126 7974 0.3 2499490 2499902 GATAATCTACTATAGAATTGAAAG 24 6 412 0.02 Sulfolobus islandicus HVE10/4 2655201 2266259 2266502 TCTAGTCTTTCAATATCTTGCTTAGTAGCCA 31 3 243 0.01 Archaeoglobus veneficus SNP6 1901943   665147   669770   GTTGAAATCAGACTAATGTAGGATTGAAAG   30   67   4623   0.24 Haloarcula marismortui ATCC 43049 chromosome I   3131724 3050405 3050562 GGCGGTCCCTGTTCGCTCTGGTT 23 3 157 0.00 Halogeometricum borinquense DSM 11551 chromosome 2820544 147680 148091 TCTGTCTCGTTCGACGACTCTGTCTCAGTGG 31 6 411 0.01 Halogeometricum borinquense DSM 11551 plasmid pHBOR01 362194 147158 147398 CTAACAGACGAAATGAGGGGTGTG 24 4 240 0.06 Haloquadratum walsbyi C23 3148033 403466 406637 GTTGCAACGAAGAGAAAACCCGCTAAGGGATTGAAAC 37 43 3171 0.10 1391076 1392481 GTTTCAGATGAACCCTTGTTGGGTTGAAGT 30 21 1405 0.04 1403321 1404737 GTTTCAGATGAACCCTTGATGGGTTGAAGT 30 21 1416 0.04 Natrialba magadii ATCC 43099 (chromosome) 3751858 1454137 1454296 GAGGTGCTGTAGTTGAGGGTGCTGTG 26 3 159 0.00 1647603 1649414 GTTCCAGAACTACCCTTGTGGGATTGAAGC 30 27 1811 0.05 Natronomonas pharaonis DSM 2160 plasmid PL131 130989 97468 97717 GCACCCCTCTATCGATGTGTACT 23 3 249 0.19

Table 1a: Novel CRISPR sequences found within the strains. a: A total of 33 new CRISPRs are identified and listed below.