Figure 1: Plot of Hydrogen atom to Oxygen atom (H:O) ratio shown as function of glycerol concentration. HT and OT represent total number of H-atoms and O-atoms available for H-bonding respectively, Ow and Og represent total number of O-atoms in water and glycerol respectively. Note the freezing point is minimum at 60% v/v glycerol concentration where HT:Ow + HT:Og is minimum [19].