Table 1

	Peptide alone	Peptide+Zn²⁺	Peptide+Mg²⁺	Peptide+Mn²⁺	Peptide+Cu²⁺
Time (min)	17.92 ± 0.01	17.69 ± 0.06*	17.88 ± 0.02*	17.85 ± 0.06	17.75 ± 0.12*

Peptide (L¹EDGPKFL⁸) (100 μg/ml) alone or with ions in a ratio 1:10 was analyzed in a C18 column. The value represents median ± SD of retention time (min) of the peptide in 20 independent runs. *p<0.01 compared with the control group

Table 1: The effect of different ions on the retention time of peptide in RP-HPLC.