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Figure 7: The shift of the oxy- / deoxymyoglobin equilibrium in the presence of
mitochondria inhibited by antimycin A (a), and lecithin liposomes (b). a) 1– MbO2 spectrum without mitochondria (the control), 2 and 3– in the presence of mitochondria at concentration of 1 and 2 mg/ml of mitochondrial protein, correspondently, 2* and 3* – the same with deduction of the light scattering, 2** and 3** –– the difference (2* minus 1 and 3* minus 1) spectra. Incubation medium as in Figure 4, antimycin A, 20 μM, pH 7.2, 22°C. b) 1–MbO2 without liposomes (the control), 2– MbO2 in the presence of 50-fold molar excess of lecithin, 2*– the same with deduction of the light scattering, 2** – the difference (2* minus 1) spectrum.Incubation medium as in Figure 4, pH 7.2, 22°C. |