Ligand form Mb
рН 7.4
рН 6.4
K
m
×10
–4
, М
–1
Quenching degree, %
K
m
×10
–4
, М
–1
Quenching degree, %
1,8-АНС
MbO
2
2.3 ± 0.3
24 ± 3
2.7 ± 0.4
20 ± 3
metMb
3.5 ± 0.4
31 ± 3
3.8 ± 0.4
21 ± 3
MbO
2
М 540
2.5 ± 0.3
23 ± 3
2.8 ± 0.3
17 ± 3
metMb
3.1 ± 0.4
37 ± 4
3.2 ± 0.4
17 ± 3
Table 2:
Quenching of the fluorescence of the 1,8-ANS and M 540 probes associated with mitochondria by MbO
2
and metMb in the pH 6-8 range (the incubation medium without succinate).
