| Influential factor |
Effect for experimental systems |
in vitrotest |
in vivotest |
|
| Adsorption ability
(Proteins, salts) |
Adsorption of molecules to particles |
Yes |
Yes |
primary effect |
| Influence of adsorption effect on experimental result.
(Starvation effect, adsorption of marker molecules, etc.) |
Yes |
No |
confounding effects
(artificial) |
| Metal ion release |
Metal ion release from nanoparticles |
Yes |
Yes |
primary effect |
| Accumulation of metal ions in experimental system |
Yes |
No |
confounding effects
(artificial) |
| Influence to experimental reaction such as ELISA |
Yes |
No |
confounding effects
(artificial) |
| Stability of nanosuspension |
Formation of secondary particles, aggregates and/or agglomerates |
Yes |
Yes |
primary effect |
| Accumulation of the large secondary particles onto cells by gravitational settling |
Yes |
No |
confounding effects
(artificial) |
