Theoretical drug loading (% w/w) Particle size (nm ) PDI Zeta potential (mV) 10 362.3 ± 12.0 0.16 ± 0.010 - 18.4 ± 1.56 20 374.1 ± 18.8 0.22 ± 0.055 - 15.2 ± 0.74 30 393.2 ± 14.91 0.19 ± 0.007 -18.7 ± 2.00

Table 2: Effect of theoretical drug loading on particle size, polydispersity (PDI), the Zeta potential, encapsulation efficiency and drug loading (DL) at PVA = 0.5 (% w/v), PLGA = 0.8 (% w/v), pH = 9).