Figure 3: HPLC chromatograms of the methanol extracts from strain KC1 cells cultured under the different light conditions, far red (FR), red, blue and white. Chlorophylls and carotenoids were extracted with 100% methanol from strain KC1 cells and were injected into an ODS reversed phase HPLC column (Spherisorb 5 μm ODS2, 4.6 x 250 mm, Waters, UK). Pigments were eluted with water-methanol-acetone (0 to 10 min linear gradient 90% to 100% methanol, 10 to 30 min 100% methanol, 30 to 31 min linear gradient methanol to acetone, 31 to 35 min 100% acetone) at a flow rate of 1.0 mL min-1 at 298 K, and were monitored with a Waters 2996 PDA detector (350- 800 nm). Chromatograms were normalized with the peak height of Chl a (peak 6). Peak 1: mixoxanthophyll, peak 2&3: unknown carotenoid, peak 4: zeaxanthin, peak 5: chlorophyll f, peak 6: chlorophyll a, peak 7 echinenone, peak 8: β-carotene.