Figure 1: Single-step affinity purification of MjK2 constructs. MjK2 with elongated fusion sites at the N- or C-terminus were bound to a Co2+-chelating column, washed with Na buffer (10 mM Tris-HCl, and 500 mM NaCl, pH 8,0) and eluted in a Na buffer supplemented with 300 mM imidazole. The purified proteins were stained with Coomassie blue (CO) or detected with an anti-X-press antibody (XP). a) (lane 1) C-terminal tagged MjK2CT (CO), (lane 2) N-terminal tagged MjK2NT (CO), and (lane 3) N-terminal tagged MjK2NT (XP) b) Effect of putative stabilizers on oligomer formation (CO). Incubation of purified untagged MjK2 in 100 mM KCl, 30 mM octyl glucoside, and 10 mM Tris at pH 8 (if not otherwise stated); (lane 1) 10 mM Ba2+; (lane 2) 10 mM Ca2+; (lane 3) 50 mM Mg-ATP; (lane 4) 10 mM K-gluconate pH 4.0; and (lane 5) POPG.