A. Electrophoresis separation of radiolabelled HC27 complexed with increasing amounts of hnRNP K and free oligo at pH 6.5 and 8. Protein/DNA complex and free probes are highlighted by arrows at the left of the figure.
B. Apparent Kd calculation plots, linear regression of hnRNP K molar concentration versus the concentration of [complex]/[free probe] ratio plot for each EMSA at pH 6.5 and 8.
