Strain Zone width (cm) Zone height (cm) Drug-resistance
L. lactis LM 0230 2.10 ±.20 1.70±.20  
  L. monocytogenes10403S 1.45±.03 1.00±.01  
  Enterococcus faecalis CK111 1.30±.10 0.60±.00  
  S. aureus SH1000 1.40±.01 1.00±.00 MSSA
  S. aureus 209P 2.50±.55 1.20±.10 MSSA
  S. aureus DU4916S 2.20±.15 1.40±.10 MSSA
S. aureus 1316 P+M- 1.20±.05 1.10±.09 MSSA
  S. aureus 1316 P-M- 2.00±.05 1.40±.20 MSSA
  S. aureus COL 1.60±.01 1.40±.10 MRSA
  S. aureus 592S 1.50±.05 1.10±.10 MRSA
  S. aureus MM66 1.50±.15 1.10±.15 HeteroVISA
  S. aureus 1316 P-M+ V5 MRSA* 1.90±.35 1.50±.30 Homo VISA
  S. aureus 1316 P-M+V20 MRSA* 1.40±.20 1.00±.00 Homo VISA
The inhibition zone was measured from the gel overlay assay after SDS gel electrophoresis. Each sample contains 15 ml of the crude lacxin from L. paracasei NRRL B-50314 and 15 ml gel loading dye. MSSA: Methicillin sensitive S. aureus, MRSA: Methicillin-Resistant S. aureus. Hetero VISA: Heterologous Vancomycin- Intermediate Methicillin-Resistant, Homo VISA: Homogeneous Vancomycin- Intermediate Methicillin-Resistant. * The zone is not very clear.
Table 1: HPLC analyses of the fermentation products by L. paracasei NRRL B-50314 from various substrates.