Substrate / Carbon source used Biosurfactant type Producer strain Finding References
Absolute ethanol Heteropolysaccharide bioemulsifier Acinetobacter calcoaceticus RAG-1 Emulsan acts as substrate for esterase enzyme [Shabtai and Gutnick, 1985]
Ethanol or
Sodium acetate or Hexadecane or Crude oil
Heteropolysaccharide bioemulsifier Acinetobacter calcoaceticus RAG-1 Emulsan producing RAG-1 capable of growing whereas emulsan deficient mutant failed to grow on crude oil [Pines and Gutnick, 1986]
Glucose Mannoprotein emulsifier Saccharomyces cerevisiae Mannoprotein acting as effective bioemulsifier [Cameron et al, 1988]
Glucose or ethanol Heteropolysaccharide bioemulsifier Acinetobacter calcoaceticus RAG-1 Esterase coding gene from RAG-1 cloned-expressed in E.coli. E.coli able to grow on triglycerides such as TAC. [Reddy et al, 1989]
Sodium acetate Polysaccharide Acinetobacter radioresistens KA53 Protein moiety essential for structure and emulsifying activity of bioemulsifier, alasan [Navon-Venezia et al, 1995]
Absolute ethanol Heteropolysaccharide bioemulsifier Acietobacter venetianus RAG-1 Exocellular protein esterase enhances emulsifying activity of emulsan. Deproteinized product, apoemulsan, failed to form/stabilize oil-water emulsions. [Bach et al, 2003]
Ethanol Heteropolysaccharide bioemulsifier Acietobacter venetianus RAG-1 his-tagged recombinant from RAG-1 formed hexadecane-in-water emulsions with 18 different polysaccharides [Bach and Gutnick, 2006]
Olive oil Lipopeptide Bacillus sp.SK320 Esterase and biosurfactant activity exhibiting Bacillus sp. and its recombinant, capable of emulsifying olive oil completely [Khanna et al, 2009]
Olive oil Lipopeptide Bacillus sp.SK320 Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release [Sekhon et al, 2011]
Table 5: Studies undertaken on biosurfactant-esterase complex.