Primer Gene target Sequence (5´→ 3´) Direction Expected Amplicon size (bp) Reference alkF alk GCICAIGARITIRKICAYAA forward 524 [19] alkR alk GCITGITGITCISWRTGICGYTG reverse ARHDf α-ARHD TTYRYITGYAIITAYCAYGGITGGG forward 329 [35] ARHDr α-ARHD AAITKYTCIGCIGSIRMYTTCCA reverse SP9f bamA CAGTACAAYTTCTACACVACBG forward 300 [32] ASP1r bamA CMATGCCGATYTCCTGRC reverse

Table 1: Oligonucleotide primers used for PCR assays.