Collection sites Salinity Collected samples Isolation technique Non-axenic isolates Axenic isolates Acetate utilising isolates Isolates lost Isolates cryopreserved Identified isolates Australia, QLD, Brisbane, rain water tank fresh 2 TAP Enrichment + FACS 12 12 12 0 12 Chlorella sp., Scenedesmus sp., Desmodesmus sp., Desmodesmus intermedius Australia, QLD, Brisbane, Nursery 1 fresh 3 TAP Enrichment + FACS 12 12 10 2 2 Chlorella sp., Chlorella sorokiniana, Micractinium pusillum Australia, QLD, Brisbane, Nursery 2 brackish/ fresh 3 TAP Enrichment + FACS 18 18 14 4 14 Chlorella sp., Chlorella sorokiniana, Micractinium pusillum, Scenedesmus sp. Australia, QLD, Brisbane, Nursery 3 fresh 1 TAP Enrichment + FACS 6 6 6 1 2 Chlorella sp., Micractinium sp. Australia, NSW, rainforest waterfall fresh 1 TAP Enrichment + FACS, Micro-manipulation 15 15 15 0 6 Chlorella sp., Chlorococcum sp., Chlamydomonas sp., Desmodesmus sp. Australia, QLD, SE Townsville, port, seaside pond salt 1 Micro-manipulation, FACS 2 2 1 2 0 No confirmed identifications Australia, QLD, Townsville, river outlet salt 4 Micro-manipulation, FACS 27 20 18 3 8 Chlorella sp., Chlorella sorokiniana, Australia, QLD, NE Townsville, pond salt 1 Micro-manipulation, FACS 13 4 4 9 0 Chlorella sp. Australia, QLD, Townsville, lake brackish 2 FACS in Liquid 9 3 5 3 0 Chlorella sp., Micractinium sp., Navicula pelliculosa sp. Australia, QLD, Gold coast, fish tank fresh 2 Dilution, FACS in Liquid 18 11 4 3 7 Stichococcus sp., Merismopedia sp., Elakatothrix sp., , Ankistrodesmus sp., Chlorella sp. Australia, QLD, UQ, pond fresh 3 Dilution, Micro-manipulation 9 5 9 0 3 Chlorella sp., Scenedesmus sp. Australia, QLD, D'Aguilar, river fresh 1 Micro-manipulation 7 7 0 1 3 Ankistrodesmus sp., Chlorella sp., Scenedesmus abundans Australia, QLD, Central coast lake (1) fresh 1 Micro-manipulation 3 1 0 0 0 Chamydomonas sp., Chlorella sp. Australia, QLD, Central coast  lake (2) fresh 1 Micro-manipulation 4 1 0 1 0 Euglena sp., Chlamydomonas sp., Chlorella sp. Australia, SA, Waikerie, Murray River fresh 1 Micro-manipulation 5 3 0 1 0 No confirmed identifications Australia, NSW, Yanga, storm water fresh 1 Micro-manipulation 4 1 0 1 0 Anabaena sp., Staurastrum sp., Coleastrum sp., Nannochloris sp. Australia, QLD, Goondiwindi, creek fresh 1 Micro-manipulation 3 0 0 0 0 Aulacoseira sp., Closterium sp.,

Table 1: Statistical analysis of algae isolation success from crude water samples. Collection sites, water characteristics, the number of water samples and strains isolated to increasing levels of purity are provided, together with their ability to utilise acetate, storage characteristics and species identification.