Fungi Biosurfactant type Solvent system Identification of functional group Developer References
Candida bombicola Sophorolipid Chloroform:Methanol:Water (65:15:2)
[54]
Diacylated lactone Not mentioned [12]
Candida lipolytica Protein-lipid-polysaccharide complex 1. Hexane:Isopropyl ether: Acetic acid (15:10:1)
2. Chloroform:Methanol:water (65:24:4)
Protein-40.2%,
Carbohydrate- 24.0%
Lipid- 19.5%
Ninhydrin-Free amino groups
Iodine vapors-Lipids
α-napthol-H2SO4  - Sugar [55]
[15]
Torulopsis petrophilum Glycolipid Chloroform:Methanol:Water (65:15:2) Not mentioned α-napthol [53]
Candida batistae Sophorolipid Chloroform:Methanol (8:2) Not mentioned Anthrone/sulfuric acid: [34] [18]
Pichia anamola Sophorolipid Chloroform:Methanol:Acetic acid (65:25:4) Not mentioned Iodine vapour and Molisch reagent [31]
Table 3: Various solvent systems and developer employed in TLC method.