Run Factor 1 A:pH Factor 2 B:substrate (g) Response 1 Ethanol (g/L)
1 5.80 160.00 74.6
2 5.80 188.28 61
3 4.80 180.00 57
4 7.21 160.00 36
5 6.80 140.00 48.2
6 4.39 160.00 59.7
7 6.80 180.00 43
8 5.80 131.72 68
9 4.80 140.00 60.1
Table 3: The combined effect of two factors i.e. pH and substrate concentration in 9 combinations. Standard order 1 represents the maximum ethanol concentration.