Run
Factor 1 A:pH
Factor 2 B:substrate (g)
Response 1 Ethanol (g/L)
1
5.80
160.00
74.6
2
5.80
188.28
61
3
4.80
180.00
57
4
7.21
160.00
36
5
6.80
140.00
48.2
6
4.39
160.00
59.7
7
6.80
180.00
43
8
5.80
131.72
68
9
4.80
140.00
60.1
Table 3:
The combined effect of two factors i.e. pH and substrate concentration in 9 combinations. Standard order 1 represents the maximum ethanol concentration.