Figure 5: Two-dimensional electrophoresis of intracellular protein extracts from Frankia alni sp. strain ACN14a exposed to NA. (a) Image of 2-DE of intracellular proteins extract from Frankia alni sp. strain ACN14a grown in BAPS medium. (b) Image of 2-DE of intracellular proteins extract from Frankia alni sp. strain ACN14a exposed to NA 60 ppm. Square and arrow with corresponding number from Table 1 indicate proteins down-regulated by the addition of NA 60 ppm, and circles and arrows with corresponding number from Table 1 indicate proteins up-regulated by the addition of NA 60 ppm. Intracellular protein extracts (200 μg) were separated on pH 4 - 7 linear IPG strips, followed by 12.5% SDS–PAGE electrophoresis.