Figure 5: TNF production 8 hours after adding Del-Immune V, Bifidim, or LPS to peritoneal macrophages of experimental or control mice. TNF concentrations were calculated by using data from 3 repeated experiments from PEM (1×107 cell/ml), which were treated with Del-Immune V or Bifidim or LPS (E. coli 0111– 4 μg/ml). Levels of cytokine production TNF were determined 24 after incubation of the cell with the formulations. Biological activity of TNF was assessed by cytotoxicity in the passaged culture of murine fibroblasts L-929. Results are mean ± SD. DIV=Del-Immune V®.