Figure 3: Activity of biosurfactants, produced by B. subtilis strains at different temperatures: A- oil spread test; B- inhibition activity against S. aureus ATCC 12600; Biosurfactants were isolated from B. subtilis strains, incubated at different temperatures: 1- strain BSB3 at 30°C; 2- strain BSB3 at 37°C; 3- strain 105 at 30°C; 4-strain 105 at 37°C; 5- strain 16k at 30°C; 6- strain 16 k at 37°C; 7- control (sterile water).