Product MW of peptides detected by HPLC (Da) MW of reported peptides (Da) Probable sequence1 Probable Function1 CM 391.1 803.5 391.1 802.5 Phe-Leu-Leu Lys-Ala-Val-Pro-Tyr-Pro-Gln ACE-I Cell Modulation UFBM - D1 113.0 803.5 391.1 802.5 Phe-Leu-Leu Lys-Ala-Val-Pro-Tyr-Pro-Gln ACE-I Cell Modulation UFBM - D7 391.1 803.5 391.1 802.5 Phe-Leu-Leu -Lys-Ala-Val-Pro-Tyr-Pro-Gln ACE-I Cell Modulation FBM    - D1 391.1 628.4 770.5 803.5 391.1 627.3 771.3 802.5 Phe-Leu-Leu Tyr-Leu-Gly-Tyr-Leu Ser-Arg-Tyr-Pro-Ser-Tyr Lys-Ala-Val-Pro-Tyr-Pro-Gln ACE-I Opioid agonist Opioid antagonist Cell Modulation FBM    - D7 391.1 451.7 628.4 770.5 803.5 391.1 904.4 627.3 771.3 802.5 Phe-Leu-Leu Thr-Val-Gln-Val-Thr-Ser-Thr-Ala-Val Tyr-Leu-Gly-Tyr-Leu Ser-Arg-Tyr-Pro-Ser-Tyr Lys-Ala-Val-Pro-Tyr-Pro-Gln ACE-I Antibacterial Opioid agonist Opioid antagonist Cell Modulation Abbreviations: Control Milk (CM); Unfermented Bifido Milk (UFBM); Fermented Bifido Milk (FBM); 24 hours after preparation (D1); seven days after storage at 4°C (D7). 1According to Kunda et al. [39].

Table 2: Peptides detected by LC-MS HPLC in milk, fermented and unfermented milk, probable sequence and function according to literature.