Figure 1: Quantification of L. reuteri in DNA extracted from Reuflor® tablets using TaqMan® labeled real-time PCR assay. Amplification curves of L. reuteri reference strains’s serial dilution DNA isolates, DNA isolate from a tablet and negative controls containing universal primers and L. reuteri TaqMan® labeled primer. 1. L. reuteri detected in a Reuflor® tablet DNA isolate, 2. L. reuteri (IMT 21493, 1.47 x 108 cfu/ml), 3. L. reuteri (IMT 21493, 1.47 x 107 cfu/ml), 4. L. reuteri (IMT 21493, 1.47 x 106 cfu/ml), 5. L. reuteri (IMT 21493, 1.47 x 105 cfu/ml), 6. L. reuteri (IMT 21493, 1.47 x 104 cfu/ml), 7. L. reuteri (IMT 21493, 1.47 x 103 cfu/ml), 8. water control containing universal primers and L. reuteri TaqMan® labeled primer. Experiments were repeated with three biological and two technical repeats.