Figure 5: Protein and mRNA levels of transcription factors DREAM, p-CREB, and NFAT activating protein (NFam1) before (fresh) and after organ culture. A and B, Incubation with KN93 decreased DREAM protein level. C and D, Immunohistochemistry revealed that p-CREB protein level was slightly increased by KN93 or XeC incubation. Staining of DREAM and p-CREB was found in the cytoplasm (lower panel, arrows), nuclei staining (blue) is shown with DAPI, magnification 20X E, XeC decreased the NFam1 mRNA levels (n = 3-5) * P < 0.05, ** P < 0.01 compared to 24 h.